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The internal lipids were then extracted by sonication for 45 min in 7-8 ml of fatresistance diet chloroform/MeOH (2:1). The chloroform/MeOH homogenate was filtered through glass wool and partitioned twice between chloroform and water. The internal lipids of whiteflies were separated into neutral lipids, free fatty acids fatresistance diet and phospholipids using a column of fatresistance diet 80 - 180 mg of Porasil Prep silica (Waters Corp., Milford, MA). The separation was achieved using a modification of a novel solvent system containing tertiarybutylmethylether (t-BME) (4). Lipid samples were applied to the column in hexane and lipid fractions eluted using the following solvents: hydrocarbons, wax esters, aldehydes (99.5:0.5 hexane/t-BME); triacylglycerols (TAG), alcohols (96:4 hexane/t-BME); free fatty acids (FFA) (100:2 hexane/acetic acid); phosphatidylethanolamine (PE) (20:4:1 t-BME/MeOH/0.001M
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