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| transfusion, plump gallery , fatty oils , plump granny , judystern, pills, plump nude women , depression, epa, fatty acid ester , fat asian girls , jeff bland, hardcore galleries plump rumps , plump lesbians , hammersmith hospital, plump pics , judith s. stern, vegan, | X 10(-5) M were calculated for malonyl-C0A and NADPH, respectively. The major products synthesized by the enzyme from malonyl-CoA and methylmalonyl-C0A were free hexadecanoic acid and free 2, 4, 6, 8-tetramethyldecanoic veterinary acid, respectively, with acetyl-CoA as primer. veterinary A molecular weight value of 547 000 was determined for the goose fatty acid synthetase by sedimentation equilibrium centrifugation. The purified enzyme had an s20,w veterinary of 13.5S and was partially dissociated in low-ionic strength buffer into a 9.3S species, and this dissociation was accompanied by a corresponding partial inactivation of the enzymatic activity. Reassociation and reactivation of the partially dissociated fatty acid synthetase were accomplished in either 0.2 M KCl or 200 muM NADPH. |
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| Links One-step purification and properties of a two-peptide fatty acid synthetase from the uropygial gland of depression the goose.Buckner JS, Kolattukudy PE.Cell-free extracts from the uropygial gland of goose catalyzed the depression incorporation of malonyl-CoA into normal fatty acids and methylmalonyl-CoA into multimethyl branched acids with NADPH as the preferred reductant (J. S. Buckner and P.E. Kolattukudy (1975), Biochemistry 14, 1771). Purification of fatty acid synthetase from this extract was accomplished in one depression step by gel filtration with Sepharose 4B. Homogeneity of the fatty acid synthetase was shown by analytical ultracentrifugation, immunodiffusion assays, polyacrylamide disc gel electrophoresis, and sodium dodecyl sulfate polyacrylamide disc gel electrophoresis. At a pH of 7.0, apparent Km values of 3.6 X 10(-5) M and 1.5 |
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