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The two studies that have quantified chitosan’s effect on fecal fat medline excretion used doses medline of chitosan close to those recommended by the manufacturers (2.7, 5.25 g/d) and found no increase in fecal fat excretion (5) (23) . In medline this study, subjects ate an average of 132 g of fat and took 4.5 g of chitosan daily. Based on the package claims (10.8 mL of fat bound per gram active ingredient), we would expect 44 g of dietary fat to be bound (10.8 mL x 0.90 g/mL x 4.5 g chitosan) and excreted in the feces. Only 1.1 g of extra fat was actually excreted in the feces. Thus, the calculated in vivo binding capacity per gram of chitosan amounts to 0.25 g of fat per gram of chitosan. To achieve a 50% reduction in fat digestibility in these subjects at this rate of binding, 264 g of this product, or 587 capsules per day, would theoretically be needed. In those studies where an increase of fecal fat was observed in rodents consuming massive quantities of chitosan, there was also an increase of fat as percent of fecal mass (19) (21) .
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